Composite

Part:BBa_K1086001

Designed by: Ítalo Faria do Valle, Marianna Kunrath Lima   Group: iGEM13_UFMG_Brazil   (2013-09-11)


RCNA + YFP

We made a composite of RCNA promoter and YFP protein in order to indirectly measure the amount of IMA (Isquemia Modified Albumin). Normal albumin binds to cobalt whereas IMA binds to cobalt in a less extent. Then if the sample has more IMA than normal albumin, there will be more free cobalt, thus more activation of the RCNA promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 47
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None